Primary structure of soybean lipoxygenase-1. [J Biol Chem. 1987]

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1: J Biol Chem. 1987 Jul 25;262(21):10080-5. Links

Primary structure of soybean lipoxygenase-1.

Shibata D, Steczko J, Dixon JE, Hermodson M, Yazdanparast R, Axelrod B.

The primary structure of lipoxygenase-1 from soybeans has been determined. The results were deduced from the nucleotide sequence of the complete cDNA for isozyme lipoxygenase-1 and were confirmed by Edman sequencing of selected peptides obtained by CNBr cleavage and supported by carboxyl-terminal sequencing of the intact protein and by partial peptide analysis employing fast atom bombardment-mass spectroscopy. The enzyme has a molecular weight of 94,038 based on its content of 838 amino acids.

PMID: 3112136 [PubMed - indexed for MEDLINE]

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