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Cloning of cDNA coding for human tissue-type plasminogen activator and its expression in Escherichia coli.
cDNA clones of the mRNA coding for tissue-type plasminogen activator (t-PA) have been obtained and their nucleotide sequences compared to those reported previously. A gene coding for t-PA has been reconstructed and inserted into vectors for expression in prokaryotic cells. Relatively high levels of t-PA accumulated in inclusion bodies in Escherichia coli containing an optimized expression plasmid, but only a small proportion of the insoluble protein was recovered as active enzyme using a variety of solubilization procedures.
PMID: 3090401 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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Secretion of active recombinant human tissue plasminogen activator derivatives in Escherichia coli.
Manosroi J, Tayapiwatana C, Götz F, Werner RG, Manosroi A.
Appl Environ Microbiol. 2001 Jun; 67(6):2657-64.
[Appl Environ Microbiol. 2001]
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Nucleotide sequence of the tissue-type plasminogen activator cDNA from human fetal lung cells.
Sasaki H, Saito Y, Hayashi M, Otsuka K, Niwa M.
Nucleic Acids Res. 1988 Jun 24; 16(12):5695.
[Nucleic Acids Res. 1988]
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Variants of human tissue-type plasminogen activator that lack specific structural domains of the heavy chain.
Gething MJ, Adler B, Boose JA, Gerard RD, Madison EL, McGookey D, Meidell RS, Roman LM, Sambrook J.
EMBO J. 1988 Sep; 7(9):2731-40.
[EMBO J. 1988]