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Alternative use of 5' exons in the specification of Ly-5 isoforms distinguishing hematopoietic cell lineages.
Previous inferences that Ly-5 glycoprotein isoforms of murine hematopoietic cells are generated by alternative splicing of primary transcripts of a single Ly-5 gene are supported by the present study. A cDNA library was prepared from B cells by extension from primer representing a known T-cell cDNA sequence. Three different Ly-5 clones from this library included sequences missing in T-cell cDNA clones. From the constitution of cDNA clones and of the Ly-5 gene, and from S1 nuclease mapping, it is concluded that at least two exons, provisionally numbered Ex-6(B) and Ex-7(B), in the 5'-proximal region are mainly represented in mRNA of the B-cell lines examined but not of the T-cell lines examined. Also, exons 1 and 2 appear to be used alternatively in different species of B-cell mRNA and probably also in different species of T-cell mRNA.
PMID: 3037546 [PubMed - indexed for MEDLINE]
PMCID: PMC298856
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Cited by 22 PubMed Central articles
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Genomic organization of the channel catfish CD45 functional gene and CD45 pseudogenes.
Kountikov E, Wilson M, Quiniou S, Miller N, Clem W, Bengtén E.
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[Immunogenetics. 2005]
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Structural and functional analysis of the human CD45 gene (PTPRC) upstream region: evidence for a functional promoter within the first intron of the gene.
Timón M, Beverley PC.
Immunology. 2001 Feb; 102(2):180-9.
[Immunology. 2001]
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MPTP delta, a putative murine homolog of HPTP delta, is expressed in specialized regions of the brain and in the B-cell lineage.
Mizuno K, Hasegawa K, Katagiri T, Ogimoto M, Ichikawa T, Yakura H.
Mol Cell Biol. 1993 Sep; 13(9):5513-23.
[Mol Cell Biol. 1993]
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