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Molecular cloning of cDNAs of human liver and placenta NADH-cytochrome b5 reductase.
A cDNA coding for human liver NADH-cytochrome b5 reductase (cytochrome b5 reductase, EC 1.6.2.2) was cloned from a human liver cDNA library constructed in phage lambda gt11. The library was screened by using an affinity-purified rabbit antibody against NADH-cytochrome b5 reductase of human erythrocytes. A cDNA about 1.3 kilobase pairs long was isolated. By using the cDNA as a probe, another cDNA (pb5R141) of 1817 base pairs was isolated that hybridized with a synthetic oligonucleotide encoding Pro-Asp-Ile-Lys-Tyr-Pro, derived from the amino acid sequence at the amino-terminal region of the enzyme from human erythrocytes. Furthermore, by using the pb5R141 as a probe, cDNA clones having more 5' sequence were isolated from a human placenta cDNA library. The amino acid sequences deduced from the nucleotide sequences of these cDNA clones overlapped each other and consisted of a sequence that completely coincides with that of human erythrocytes and a sequence of 19 amino acid residues extended at the amino-terminal side. The latter sequence closely resembles that of the membrane-binding domain of steer liver microsomal enzyme.
PMID: 3035541 [PubMed - indexed for MEDLINE]
PMCID: PMC304924
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Cited by 3 PubMed Central articles
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cDNA Clones for Corn Leaf NADH:Nitrate Reductase and Chloroplast NAD(P):Glyceraldehyde-3-Phosphate Dehydrogenase : Characterization of the Clones and Analysis of the Expression of the Genes in Leaves as Influenced by Nitrate in the Light and Dark.
Gowri G, Campbell WH.
Plant Physiol. 1989 Jul; 90(3):792-798.
[Plant Physiol. 1989]
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Two transcripts encode rat cytochrome b5 reductase.
Pietrini G, Carrera P, Borgese N.
Proc Natl Acad Sci U S A. 1988 Oct; 85(19):7246-50.
[Proc Natl Acad Sci U S A. 1988]
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A single mRNA, transcribed from an alternative, erythroid-specific, promoter, codes for two non-myristylated forms of NADH-cytochrome b5 reductase.
Pietrini G, Aggujaro D, Carrera P, Malyszko J, Vitale A, Borgese N.
J Cell Biol. 1992 Jun; 117(5):975-86.
[J Cell Biol. 1992]