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cDNA cloning and mapping of the human creatine kinase M gene to 19q13.
Nigro JM,
Schweinfest CW,
Rajkovic A,
Pavlovic J,
Jamal S,
Dottin RP,
Hart JT,
Kamarck ME,
Rae PM,
Carty MD, et al.
We describe the first isolation of a human creatine kinase M cDNA clone and its mapping of the gene to human chromosome 19. A human creatine kinase M cDNA clone, pJN2CK-M, harboring a 1,160-bp insert, was isolated by colony hybridization with a previously sequenced chicken creatine kinase M cDNA probe. The human cDNA was used as a probe in Southern transfers of TaqI-digested genomic DNA from mouse/human somatic-cell hybrids to localize the human creatine kinase-M gene to chromosome 19. In situ hybridization of the tritiated cDNA probe to metaphase chromosomes of peripheral blood lymphocytes from normal males revealed significant labeling to chromosome 19. These two independent methodologies assign the human creatine kinase-M gene to chromosome 19. Since greater than 69% of the grains of chromosome 19 label band q13, the human creatine kinase-M gene has been mapped to 19q13. On the basis of high-resolution G-banding, the predominant labeling site was 19q13.2-q13.3.
PMID: 3031982 [PubMed - indexed for MEDLINE]
PMCID: PMC1684079
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Cited by 7 PubMed Central articles
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Post-infarction left ventricular remodeling induces changes in creatine kinase mRNA and protein subunit levels in porcine myocardium.
Hoang CD, Zhang J, Payne RM, Apple FS.
Am J Pathol. 1997 Jul; 151(1):257-64.
[Am J Pathol. 1997]
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Human creatine kinase genes on chromosomes 15 and 19, and proximity of the gene for the muscle form to the genes for apolipoprotein C2 and excision repair.
Stallings RL, Olson E, Strauss AW, Thompson LH, Bachinski LL, Siciliano MJ.
Am J Hum Genet. 1988 Aug; 43(2):144-51.
[Am J Hum Genet. 1988]
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Carcinoembryonic antigen family: expression in a mouse L-cell transfectant and characterization of a partial cDNA in bacteriophage lambda gt11.
Kamarck ME, Elting JJ, Hart JT, Goebel SJ, Rae PM, Nothdurft MA, Nedwin JJ, Barnett TR.
Proc Natl Acad Sci U S A. 1987 Aug; 84(15):5350-4.
[Proc Natl Acad Sci U S A. 1987]
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