-
Isolation and characterization of human blood-coagulation factor X cDNA.
Using synthetic oligodeoxynucleotides as probes, we have isolated factor X cDNA from human liver cDNA library. We sequenced the 1430-bp cDNA which spans the coding region of the mature factor X and contains the polyadenylation signal and poly(A) tail. The amino acid (aa) sequence is in agreement with the published aa sequence. The nucleotide (nt) sequence of cDNA confirmed that factor X is synthesized and secreted as a single-chain precursor, and then converted into dimeric form by proteolytic cleavage of an internal tripeptide. From the nt sequence, it was also predicted that like other secretory proteins, human factor X is synthesized with a leader sequence (prepro-protein). The 5'-coding region of factor X cDNA is 60 and 40% homologous to the corresponding regions of factor IX and prothrombin genes, respectively. This supports the hypothesis of gene evolution by gene duplication followed by divergence.
PMID: 3011603 [PubMed - indexed for MEDLINE]
-
Cited by 2 PubMed Central articles
-
Isolation of cDNA clones coding for human tissue factor: primary structure of the protein and cDNA.
Spicer EK, Horton R, Bloem L, Bach R, Williams KR, Guha A, Kraus J, Lin TC, Nemerson Y, Konigsberg WH.
Proc Natl Acad Sci U S A. 1987 Aug; 84(15):5148-52.
[Proc Natl Acad Sci U S A. 1987]
-
Factor XSanto Domingo. Evidence that the severe clinical phenotype arises from a mutation blocking secretion.
Watzke HH, Wallmark A, Hamaguchi N, Giardina P, Stafford DW, High KA.
J Clin Invest. 1991 Nov; 88(5):1685-9.
[J Clin Invest. 1991]