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Organization of the human genes for insulin-like growth factors I and II.
Recently, we have reported the isolation of cDNAs encoding the precursors of insulin-like growth factors I and II (IGF-I and II) [(1983) Nature 306, 609-611; (1985) FEBS Lett. 179, 243-246. These cDNAs were employed as specific probes to detect and isolate the corresponding genes from human cosmid DNA libraries. Three cosmids were detected, together containing the entire cDNA sequence of IGF-I, and one cosmid containing the sequence of IGF-II cDNA. Southern blot hybridization, physical mapping and nucleotide sequence analysis of these cosmids revealed that the IGF-I and -II genes have a discontinous structure. The IGF-I gene contains at least four exons spanning a region of probably more that 45 kilobasepairs (kb), while the IGF-II gene consists of at least five exons, spanning a region of 16 kb.
PMID: 3002851 [PubMed - indexed for MEDLINE]
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Cited by 6 PubMed Central articles
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Genetic and environmental components of interindividual variation in circulating levels of IGF-I, IGF-II, IGFBP-1, and IGFBP-3.
Harrela M, Koistinen H, Kaprio J, Lehtovirta M, Tuomilehto J, Eriksson J, Toivanen L, Koskenvuo M, Leinonen P, Koistinen R, et al.
J Clin Invest. 1996 Dec 1; 98(11):2612-5.
[J Clin Invest. 1996]
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Translation initiation on the insulin-like growth factor II leader 1 is developmentally regulated.
Teerink H, Kasperaitis MA, De Moor CH, Voorma HO, Thomas AA.
Biochem J. 1994 Oct 15; 303 ( Pt 2):547-53.
[Biochem J. 1994]
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Tissue-specific expression of insulin-like growth factor II mRNAs with distinct 5' untranslated regions.
Irminger JC, Rosen KM, Humbel RE, Villa-Komaroff L.
Proc Natl Acad Sci U S A. 1987 Sep; 84(18):6330-4.
[Proc Natl Acad Sci U S A. 1987]
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