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Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta.
Bacillus thuringiensis subsp. kurstaki HD-73 produces a crystal protein which is lethal to many lepidopteran larvae. The gene encoding this crystal protein has been isolated from a 75-kb plasmid and engineered into a recombinant Escherichia coli plasmid for analysis. The complete nucleotide sequences of the coding region and 387-bp 5' and 376-bp 3' to the coding region have been determined. The 3537-bp of the coding region specify a protein of Mr 133 330. The full-length gene and several 3' -truncated derivatives of the gene were examined in both E. coli and in an E. coli minicell-expression system to determine if the carboxy end of the protein is essential for toxicity. The results presented here provide the primary structure of the crystal protein gene and show that the N-terminal 68-kDal peptide is toxic, but at a lower level than the full-length gene product.
PMID: 3000881 [PubMed - indexed for MEDLINE]
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Cited by 44 PubMed Central articles
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Cloning and expression of two crystal protein genes, cry30Ba1 and cry44Aa1, obtained from a highly mosquitocidal strain, Bacillus thuringiensis subsp. entomocidus INA288.
Ito T, Ikeya T, Sahara K, Bando H, Asano S.
Appl Environ Microbiol. 2006 Aug; 72(8):5673-6.
[Appl Environ Microbiol. 2006]
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Molecular cloning and characterization of the insecticidal crystal protein gene of Bacillus thuringiensis var. tenebrionis.
Sekar V, Thompson DV, Maroney MJ, Bookland RG, Adang MJ.
Proc Natl Acad Sci U S A. 1987 Oct; 84(20):7036-7040.
[Proc Natl Acad Sci U S A. 1987]
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Binding of Bacillus thuringiensis Cry1 Toxins to the Midgut Brush Border Membrane Vesicles of Chilo suppressalis (Lepidoptera: Pyralidae): Evidence of Shared Binding Sites.
Fiuza L, Nielsen-Leroux C, Goze E, Frutos R, Charles J.
Appl Environ Microbiol. 1996 May; 62(5):1544-1549.
[Appl Environ Microbiol. 1996]
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