-
Cloning and characterization of the cDNAs for human and rabbit interleukin-1 precursor.
DNA sequence complementary to the mRNA for rabbit interleukin-1 precursor (preIL-1) has been cloned from the cDNA library constructed using partially purified poly(A)+RNA from induced rabbit alveolar macrophages by mRNA hybridization-translation assay. By using this cDNA as a probe, human IL-1 cDNA was isolated from the cDNA library prepared using poly(A)+RNA from induced HL-60 cells, a human monocyte-like cell line. The amino acid sequences of the human and rabbit preIL-1 deduced from the cDNA sequences reveal their primary structures which consists of 271 and 267 amino acid residues, respectively. The amino acid sequence is 64% conserved between human and rabbit. The difference in number of amino acid residues results from the carboxy-terminal extention of 4 amino acid residues in human preIL-1. Expression of the cloned human cDNA in E. coli yielded biologically active IL-1.
PMID: 2994016 [PubMed - indexed for MEDLINE]
PMCID: PMC321918
-
Cited by 34 PubMed Central articles
-
Regulation of electrolyte transport with IL-1beta in rabbit distal colon.
Homaidan FR, Desai H, Zhao L, Broutman G, Burakoff R.
Mediators Inflamm. 1995; 4(1):61-6.
[Mediators Inflamm. 1995]
-
Increased interleukin-1 (IL-1) and imbalance between IL-1 and IL-1 receptor antagonist during acute inflammation in experimental Shigellosis.
Arondel J, Singer M, Matsukawa A, Zychlinsky A, Sansonetti PJ.
Infect Immun. 1999 Nov; 67(11):6056-66.
[Infect Immun. 1999]
-
Enhancement by galactosamine of lipopolysaccharide(LPS)-induced tumour necrosis factor production and lethality: its suppression by LPS pretreatment.
Endo Y, Shibazaki M, Yamaguchi K, Kai K, Sugawara S, Takada H, Kikuchi H, Kumagai K.
Br J Pharmacol. 1999 Sep; 128(1):5-12.
[Br J Pharmacol. 1999]
- » See all...