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Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase.
The yeast GDH1 gene encodes NADP-dependent glutamate dehydrogenase. This gene was isolated by complementation of an Escherichia coli glutamate auxotroph. NADP-dependent glutamate dehydrogenase was overproduced 6-10-fold in Saccharomyces cerevisiae bearing GDH1 on a multicopy plasmid. The nucleotide sequence of the 1362-base pair coding region and 5' and 3' flanking sequences were determined. Transcription start sites were located by S1 nuclease mapping. Regulation of GDH1 was not maintained when the gene was present on a multicopy plasmid. Protein secondary structure predictions identified a region with potential to form the dinucleotide-binding domain. The amino acid sequences of the yeast and Neurospora crassa enzymes are 63% conserved. Unlike the N. crassa gene, yeast GDH1 has no introns.
PMID: 2989290 [PubMed - indexed for MEDLINE]
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Cited by 9 PubMed Central articles
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Expression of the Escherichia coli pntA and pntB genes, encoding nicotinamide nucleotide transhydrogenase, in Saccharomyces cerevisiae and its effect on product formation during anaerobic glucose fermentation.
Anderlund M, Nissen TL, Nielsen J, Villadsen J, Rydström J, Hahn-Hägerdal B, Kielland-Brandt MC.
Appl Environ Microbiol. 1999 Jun; 65(6):2333-40.
[Appl Environ Microbiol. 1999]
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GDH3 encodes a glutamate dehydrogenase isozyme, a previously unrecognized route for glutamate biosynthesis in Saccharomyces cerevisiae.
Avendaño A, Deluna A, Olivera H, Valenzuela L, Gonzalez A.
J Bacteriol. 1997 Sep; 179(17):5594-7.
[J Bacteriol. 1997]
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Arabidopsis mutant analysis and gene regulation define a nonredundant role for glutamate dehydrogenase in nitrogen assimilation.
Melo-Oliveira R, Oliveira IC, Coruzzi GM.
Proc Natl Acad Sci U S A. 1996 May 14; 93(10):4718-23.
[Proc Natl Acad Sci U S A. 1996]
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