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Complete characterization of the human IGF-I nucleotide sequence isolated from a newly constructed adult liver cDNA library.
A full-size cDNA sequence coding for insulin-like growth factor I (IGF-I) was isolated from a human liver library. For the construction of this bank, a new method was developed which anneals dG-tailed cDNA with a synthetic adaptor 5'-AATTCCCCCCCCCCC-3' followed by ligation into the EcoRI site of a lambda immunity-insertion vector. Based on the sequence analysis of the complete IGF-I messenger we concluded that the protein is synthesized as a precursor containing a signal peptide of 22 or 25 amino acid residues. In addition, the sequence extended in the 3'-direction and showed the presence of multiple polyadenylation sites in the IGF-I message.
PMID: 2935423 [PubMed - indexed for MEDLINE]
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Cited by 5 PubMed Central articles
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Large deletion of the peroxisomal acyl-CoA oxidase gene in pseudoneonatal adrenoleukodystrophy.
Fournier B, Saudubray JM, Benichou B, Lyonnet S, Munnich A, Clevers H, Poll-The BT.
J Clin Invest. 1994 Aug; 94(2):526-31.
[J Clin Invest. 1994]
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Molecular characterization of the testis specific c-abl mRNA in mouse.
Meijer D, Hermans A, von Lindern M, van Agthoven T, de Klein A, Mackenbach P, Grootegoed A, Talarico D, Della Valle G, Grosveld G.
EMBO J. 1987 Dec 20; 6(13):4041-8.
[EMBO J. 1987]
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Use of human surfactant low molecular weight apoproteins in the reconstitution of surfactant biologic activity.
Revak SD, Merritt TA, Degryse E, Stefani L, Courtney M, Hallman M, Cochrane CG.
J Clin Invest. 1988 Mar; 81(3):826-33.
[J Clin Invest. 1988]
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