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1: Biochem Biophys Res Commun. 1989 Feb 15;158(3):991-5.Links

The nucleotide sequence of a full length cDNA clone encoding rat liver urate oxidase.

Alvares K, Nemali MR, Reddy PG, Wang XD, Rao MS, Reddy JK.

Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611.

Recently we reported the sequence of a cDNA clone (pUOX-1), isolated from a lambda gt11 cDNA library, which encoded for rat liver urate oxidase (EC 1.7.3.3), but this clone lacked the nucleotide sequences encoding the N-terminal region for this enzyme. Using the cDNA insert from the pUOX-1 clone as a probe, we have now isolated a full length cDNA clone, pUOX-2, from a lambda gt10 library by plaque hybridization. Nucleotide sequence analysis of the pUOX-2 clone showed that it has 1379 base pairs with an open reading frame coding for 303 amino acid residues corresponding to a molecular mass of 34,931 daltons. In addition to the open reading frame the pUOX-2 contains 439 bp of 3'-untranslated and 41 bp of 5'-untranslated sequences. The consensus polyadenylation signal AATAAA precedes a stretch of poly(A)+ residues at the 3' end.

PMID: 2920046 [PubMed - indexed for MEDLINE]