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cDNA cloning and nucleotide sequence of rat muscle-specific enolase (beta beta enolase).
Department of Biology, Faculty of Science, Niigata University, Japan.
The nucleotide sequence of rat muscle-specific enolase cDNA was determined by sequencing three cDNA clones encoding this enolase isozyme. The nearly full-length cDNA consists of 13-bp 5'- and 84-bp 3'-noncoding regions and a poly(A) tail in addition to a 1302-bp coding region encoding a polypeptide composed of 434 amino acid residues. The deduced primary structure of this enolase isozyme is about 80% similar to those determined previously for rat neuron-specific and non-neuronal enolase isozymes. Southern blot analysis suggested strongly the existence of a single copy of the muscle-specific enolase gene per haploid genome. The mRNA for this enolase isozyme was detected in rat skeletal muscle on day 1 after birth and its level increased rapidly during 10-30 days after birth without any change in its size (1500 bases).
PMID: 2914621 [PubMed - indexed for MEDLINE]
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Cited by 6 PubMed Central articles
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Molecular cloning and characterization of the Candida albicans enolase gene.
Mason AB, Buckley HR, Gorman JA.
J Bacteriol. 1993 May; 175(9):2632-9.
[J Bacteriol. 1993]
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Murine muscle-specific enolase: cDNA cloning, sequence, and developmental expression.
Lamandé N, Mazo AM, Lucas M, Montarras D, Pinset C, Gros F, Legault-Demare L, Lazar M.
Proc Natl Acad Sci U S A. 1989 Jun; 86(12):4445-9.
[Proc Natl Acad Sci U S A. 1989]
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New nucleotide sequence data on the EMBL File Server.
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Nucleic Acids Res. 1990 Apr 11; 18(7):1933-7.
[Nucleic Acids Res. 1990]
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