-
Structure of the murine lck gene and its rearrangement in a murine lymphoma cell line.
Howard Hughes Medical Institute, University of Washington, Seattle 98195.
The lck gene encodes a lymphocyte-specific protein-tyrosine kinase that is implicated in neoplastic transformation. We have determined the germ line organization of the murine lck gene and have isolated and characterized a rearranged lck allele in the murine lymphoma cell line LSTRA. The overall exon-intron organization of the normal lck gene is almost identical to that of avian c-src. In LSTRA DNA, an internally rearranged Moloney murine leukemia virus genome is interposed between two distinct promoters that normally generate lck transcripts differing only in 5' untranslated regions. The rearrangement appears to have been selected to permit splicing of transcripts that initiate from the Moloney virus promoter to an acceptor site located within the first exon 3' to the downstream promoter, thus generating an lck mRNA with a novel 5' untranslated region that may be more efficiently translated.
PMID: 2850479 [PubMed - indexed for MEDLINE]
PMCID: PMC363532
-
Cited by 29 PubMed Central articles
-
Large-scale identification of disease genes involved in acute myeloid leukemia.
Erkeland SJ, Valkhof M, Heijmans-Antonissen C, van Hoven-Beijen A, Delwel R, Hermans MH, Touw IP.
J Virol. 2004 Feb; 78(4):1971-80.
[J Virol. 2004]
-
Conserved regulation of the lymphocyte-specific expression of lck in the Fugu and mammals.
Brenner S, Venkatesh B, Yap WH, Chou CF, Tay A, Ponniah S, Wang Y, Tan YH.
Proc Natl Acad Sci U S A. 2002 Mar 5; 99(5):2936-41. Epub 2002 Feb 26.
[Proc Natl Acad Sci U S A. 2002]
-
Lck domains differentially contribute to pre-T cell receptor (TCR)- and TCR-alpha/beta-regulated developmental transitions.
Lin K, Longo NS, Wang X, Hewitt JA, Abraham KM.
J Exp Med. 2000 Feb 21; 191(4):703-16.
[J Exp Med. 2000]
- » See all...