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Cloning, molecular characterization and chromosome localization of the inorganic pyrophosphatase (PPA) gene from S. cerevisiae.
University of Pennsylvania, Department of Chemistry, Philadelphia 19104.
The gene for Saccharomyces cerevisiae inorganic pyrophosphatase, PPA, has been cloned by hybridization of "long" oligonucleotide probes with both cDNA and genomic S. cerevisiae libraries. The nucleotide sequence of 1612 bp from a genomic subclone that includes the entire coding region gives a deduced amino acid sequence that has nine differences (out of a total of 286 residues) from the previously published amino acid sequence that was determined directly. The codon usage in PPA is as expected for a "highly expressed" yeast gene. The upstream region contains a poly dA/dT sequence that might comprise a constitutive promoter. The PPA gene appears to be present in a single copy within the S. cerevisiae genome and has been localized to chromosome II.
PMID: 2849749 [PubMed - indexed for MEDLINE]
PMCID: PMC338916
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Cited by 6 PubMed Central articles
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Functional complementation of yeast cytosolic pyrophosphatase by bacterial and plant H+-translocating pyrophosphatases.
Perez-Castineira JR, Lopez-Marques RL, Villalba JM, Losada M, Serrano A.
Proc Natl Acad Sci U S A. 2002 Dec 10; 99(25):15914-9. Epub 2002 Nov 25.
[Proc Natl Acad Sci U S A. 2002]
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Mechanism by which metal cofactors control substrate specificity in pyrophosphatase.
Zyryanov AB, Shestakov AS, Lahti R, Baykov AA.
Biochem J. 2002 Nov 1; 367(Pt 3):901-6.
[Biochem J. 2002]
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Crystal structure of inorganic pyrophosphatase from Thermus thermophilus.
Teplyakov A, Obmolova G, Wilson KS, Ishii K, Kaji H, Samejima T, Kuranova I.
Protein Sci. 1994 Jul; 3(7):1098-107.
[Protein Sci. 1994]
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