Characterization of plasmid pAZ1 and the type III ...[Plasmid. 1988]

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1: Plasmid. 1988 Jan;19(1):30-8.Links

Characterization of plasmid pAZ1 and the type III dihydrofolate reductase gene.

Fling ME, Kope J, Richards C.

Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709.

The plasmid pAZ1, which determines trimethoprim and sulfonamide resistance, was characterized by restriction endonuclease mapping. The restriction map was identical to that of the incQ plasmid RSF1010 over a 5.1-kbp region. The type III dihydrofolate reductase gene was cloned, and the DNA sequence was determined. The predicted protein had 162 amino acid residues, and it was more closely related to the gram-negative bacterial chromosomal dihydrofolate reductases than to other plasmid or vertebrate dihydrofolate reductases. Sequence identity was 51% with the Escherichia coli enzyme and 44% with the Neisseria gonorrhoeae enzyme.

PMID: 2840679 [PubMed - indexed for MEDLINE]

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[Microbiol Mol Biol Rev. 2001]
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[Antimicrob Agents Chemother. 1993]
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Trimethoprim (Proloprim® )
Trimethoprim eliminates bacteria that cause urinary tract infections. It is used in combination with other drugs to treat certain types of pneumonia. It also is used to treat 'travelers' diarrhea.' Antibiotics will not w...
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Cited by 10 PubMed Central articles

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