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1: Cell. 1988 Jul 15;54(2):275-83.Click here to read Links

SV40 large tumor antigen forms a specific complex with the product of the retinoblastoma susceptibility gene.

DeCaprio JA, Ludlow JW, Figge J, Shew JY, Huang CM, Lee WH, Marsilio E, Paucha E, Livingston DM.

Division of Neoplastic Disease Mechanisms, Dana-Farber Cancer Institute, Boston, Massachusetts.

Monkey cells synthesizing SV40 large T antigen were lysed and the extracts immunoprecipitated with either monoclonal anti-T antibody or monoclonal antibody to p110-114, the product of the retinoblastoma susceptibility gene (Rb). T and p110-114 coprecipitated in each case, implying that the proteins are complexed with each other. Substitution and internal deletion mutants of T that contain structural alterations in a ten residue, transformation-controlling domain failed to complex with p110-114. In contrast, T mutants bearing structural changes outside of this domain bound to p110-114. These results are consistent with a model for transformation by SV40 which, at least in part, involves T/p110-114 complex formation and the perturbation of Rb protein and/or T function.

PMID: 2839300 [PubMed - indexed for MEDLINE]