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Identification of a cDNA clone for the beta-subunit of the pyruvate dehydrogenase component of human pyruvate dehydrogenase complex.
Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
We report the isolation of a 1.5 kb cDNA clone for the beta subunit of human pyruvate dehydrogenase (E1) from a human liver lambda gt11 cDNA library using anti-E1 serum. We generated a peptide sequence of 24 amino acids starting from the N-terminus of bovine heart mature E1 beta. The identity of the E1 beta cDNA clone was confirmed by the similarity between the amino acid sequence deduced from the cDNA nucleotide sequence and the known amino acid sequence of bovine heart E1 beta. In Northern analysis of total RNA extracted from human heart, the E1 beta cDNA clone hybridized to a major 1.6 kb and a minor 5.2 kb RNA species.
PMID: 2829898 [PubMed - indexed for MEDLINE]
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Cited by 2 PubMed Central articles
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Pretranslational regulation of pyruvate dehydrogenase complex subunits in white adipose tissue during the suckling-weaning transition in the rat.
Maury J, Kerbey AL, Priestman DA, Patel MS, Girard J, Ferre P.
Biochem J. 1995 Oct 15; 311 ( Pt 2):531-5.
[Biochem J. 1995]
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Heterogeneous expression of protein and mRNA in pyruvate dehydrogenase deficiency.
Wexler ID, Kerr DS, Ho L, Lusk MM, Pepin RA, Javed AA, Mole JE, Jesse BW, Thekkumkara TJ, Pons G.
Proc Natl Acad Sci U S A. 1988 Oct; 85(19):7336-40.
[Proc Natl Acad Sci U S A. 1988]