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Expression of human uterine tissue-type plasminogen activator in mouse cells using BPV vectors.
Integrated Genetics, 31 New York Avenue, Framingham, MA 01701.
Human tissue-type plasminogen activator (t-PA) cDNA was cloned from uterine tissue and engineered in expression vectors for production in mouse C127 cells. The vectors consisted of the bovine papilloma virus-1 (BPV-1) genome and t-PA transcriptional unit with a mouse metallothionein (MT-1) promoter at the 5' end and MT-1 genomic sequences or SV40 early introns and polyadenylation signals at the 3' end. Analysis of the expression vectors transfected into cells revealed that t-PA is expressed 100- to 200-fold more with an intronless vector utilizing the SV40 polyadenylation signal than with other, intron-containing vectors. RNA analysis of stable cell lines indicated that t-PA expression levels correlated with mRNA abundance. DNA copy number and transcriptional rate of the MT-1 promoter remained constant in cell lines transformed by different BPV expression vectors. Uterine t-PA produced by recombinant DNA means was enzymatically active and similar in properties to Bowes melanoma t-PA.
PMID: 2824147 [PubMed - indexed for MEDLINE]
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Cited by 4 PubMed Central articles
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Upregulation of swelling-activated Cl- channel sensitivity to cell volume by activation of EGF receptors in murine mammary cells.
Abdullaev IF, Sabirov RZ, Okada Y.
J Physiol. 2003 Jun 15; 549(Pt 3):749-58. Epub 2003 Apr 17.
[J Physiol. 2003]
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The glycosylation of Bowes melanoma tissue plasminogen activator: lectin mapping, reaction with anti-L2/HNK-1 antibodies and the presence of sulphated/glucuronic acid containing glycans.
Jaques AJ, Opdenakker G, Rademacher TW, Dwek RA, Zamze SE.
Biochem J. 1996 Jun 1; 316 ( Pt 2):427-37.
[Biochem J. 1996]
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Molecular basis of ornithine aminotransferase deficiency in B-6-responsive and -nonresponsive forms of gyrate atrophy.
Ramesh V, McClatchey AI, Ramesh N, Benoit LA, Berson EL, Shih VE, Gusella JF.
Proc Natl Acad Sci U S A. 1988 Jun; 85(11):3777-80.
[Proc Natl Acad Sci U S A. 1988]
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