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Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3.
Institut für Biochemie, Technische Hochschule Darmstadt, FRG.
A cDNA library from Aspergillus niger strain NRRL-3 enriched in sequences glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12,000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5'- and a longer 3'-noncoding region. The deduced protein sequence was verified by partial peptide sequencing.
PMID: 2792372 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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The first step in polyethylene glycol degradation by sphingomonads proceeds via a flavoprotein alcohol dehydrogenase containing flavin adenine dinucleotide.
Sugimoto M, Tanabe M, Hataya M, Enokibara S, Duine JA, Kawai F.
J Bacteriol. 2001 Nov; 183(22):6694-8.
[J Bacteriol. 2001]
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Disease resistance conferred by expression of a gene encoding H2O2-generating glucose oxidase in transgenic potato plants.
Wu G, Shortt BJ, Lawrence EB, Levine EB, Fitzsimmons KC, Shah DM.
Plant Cell. 1995 Sep; 7(9):1357-68.
[Plant Cell. 1995]
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New nucleotide sequence data on the EMBL File Server.
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Nucleic Acids Res. 1989 Nov 25; 17(22):9511-24.
[Nucleic Acids Res. 1989]