Your browser version may not work well with NCBI's Web applications. More information here...
1: FEBS Lett. 1989 Sep 11;255(1):105-10.Click here to read Links

Primary structures of new 'iso-hirudins'.

Scharf M, Engels J, Tripier D.

Institut für organische Chemie, Johann Wolfgang v. Goethe Universität, Frankfurt, FRG.

Crude hirudin (12.7 U/micrograms), a complex mixture of polypeptides obtained from the leech, could be separated by microbore HPLC. A combination of amino acid analysis, N-terminal microsequencing and chemical as well as enzymatic fragmentation made the primary sequence of the new isohirudins Ia-IIIb' accessible. The biological activity determined in the thrombin inhibition test showed a comparable value for all of these compounds. The results presented address the question as to whether these isohirudins are true mutations from a family of genes or a family of leeches.

PMID: 2792365 [PubMed - indexed for MEDLINE]