Your browser version may not work well with NCBI's Web applications. More information here...
1: Gene. 1989 May 30;78(2):215-24.Links

Zeta-crystallin, a novel protein from the guinea pig lens is related to alcohol dehydrogenases.

Rodokanaki A, Holmes RK, Borrás T.

Laboratory of Mechanisms of Ocular Diseases, National Eye Institute, Bethesda, MD 20892.

zeta-Crystallin is a major component of the water-soluble proteins of the guinea pig lens. We have constructed a lens cDNA library from one- to seven-day-old guinea pigs in the plasmid Bluescript KS+ and used the 16 amino acid (aa) sequence of a CNBr peptide to design an oligodeoxyribonucleotide probe. Analysis of two positive clones and direct sequence of the 5' end of the RNA resulted in the completion of a most probably full-length mRNA comprising 1842 nucleotides (nt). The ATG start codon occurs 83 nt downstream from the 5' end. The open reading frame, ending with a stop codon at nt position 1070, predicts a protein of 328 aa with a calculated Mr of 35,071. Comparison of the amino acid sequence with the National Biomedical Research Foundation protein data base reveals a significant similarity of zeta-crystallin with the enzyme of the alcohol dehydrogenase family.

PMID: 2777081 [PubMed - indexed for MEDLINE]