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The yeast pyruvate kinase gene does not contain a string of non-preferred codons: revised nucleotide sequence.
Department of Biochemistry, University of Edinburgh, Scotland.
The sequence of the gene encoding pyruvate kinase from Saccharomyces cerevisiae was re-determined because of failures with oligonucleotide-directed mutagenesis experiments involving a region thought to contain a string of five contiguous non-preferred codons. This region was found to be difficult to sequence and was shown to have three extra bases when compared with the published sequence [(1983) J. Biol. Chem. 258, 2193-2201]. The revised sequence demonstrates that the yeast pyruvate kinase gene does not have a cluster of non-preferred codons, and that it therefore is not an example of the class of genes which possibly exhibit translational control by the presence of non-preferred codons.
PMID: 2653861 [PubMed - indexed for MEDLINE]
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Cited by 9 PubMed Central articles
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[J Bacteriol. 1998]
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Mck1, a member of the glycogen synthase kinase 3 family of protein kinases, is a negative regulator of pyruvate kinase in the yeast Saccharomyces cerevisiae.
Brazill DT, Thorner J, Martin GS.
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[J Bacteriol. 1997]
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Boles E, Schulte F, Miosga T, Freidel K, Schlüter E, Zimmermann FK, Hollenberg CP, Heinisch JJ.
J Bacteriol. 1997 May; 179(9):2987-93.
[J Bacteriol. 1997]
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