Complete sequence of the coding region of human el...[Biol Chem Hoppe Seyler. 1989]

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1: Biol Chem Hoppe Seyler. 1989 Oct;370(10):1071-5.Links

Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells.

Rapp G, Klaudiny J, Hagendorff G, Luck MR, Scheit KH.

Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Biologie, Göttingen.

The use of two primers allowed the specific enzymatic amplification of elongation factor 2 starting with total double-stranded cDNA from human ovarian granulosa cells. The amplified DNA fragment with a length of 1765 bp was restricted and sequenced by the shot gun approach. From the sequences obtained from the amplified fragment and the cDNA insert of pHGR81 [Rapp et al. (1988) Biol. Chem. Hoppe-Seyler 369, 247-250] respectively, the DNA sequence containing the complete coding as well as the 3'-untranslated region was assembled.

PMID: 2610926 [PubMed - indexed for MEDLINE]

Construction of a plasmid containing the complete coding region of human elongation factor 2.
Biol Chem Hoppe Seyler. 1992 Apr; 373(4):201-4.

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Cloning and sequence analysis of a cDNA from human ovarian granulosa cells encoding the C-terminal part of human elongation factor 2.
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[Bioorg Khim. 1989]
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Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells.

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Cited by 4 PubMed Central articles

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