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Isolation of duplicated human c-src genes located on chromosomes 1 and 20.
The oncogene (v-src) of Rous sarcoma virus apparently arose by transduction of the chicken gene known as c-src(chicken). We isolated DNA fragments representative of two src-related loci from recombinant DNA bacteriophage libraries of the human genome. One of these loci, c-src1(human), appeared to direct the synthesis of a 5-kilobase polyadenylated RNA that presumably encodes pp60c-src(human). Probes specific for the other locus, c-src2(human), did not hybridize to polyadenylated RNA prepared from a variety of human cell lines. Partial nucleotide sequence determinations of the loci demonstrated that c-src1(human) is highly related to chicken c-src and that c-src2(human) is slightly more divergent. The sequences imply that the final two coding exons of each human locus are identical in length to those of chicken c-src and that the location of an amber stop codon is unchanged in all three loci. c-src1(human) has been mapped to chromosome 20, and the second locus is located on chromosome 1. We conclude that c-src1(human) is the analog of c-src(chicken) and that the duplicated locus, c-src2(human), may also be expressed.
PMID: 2581127 [PubMed - indexed for MEDLINE]
PMCID: PMC366788
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Cited by 22 PubMed Central articles
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yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family.
Semba K, Nishizawa M, Miyajima N, Yoshida MC, Sukegawa J, Yamanashi Y, Sasaki M, Yamamoto T, Toyoshima K.
Proc Natl Acad Sci U S A. 1986 Aug; 83(15):5459-63.
[Proc Natl Acad Sci U S A. 1986]
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ReviewChromosome 1 in relation to human disease.
Povey S, Parrington JM.
J Med Genet. 1986 Apr; 23(2):107-15.
[J Med Genet. 1986]
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Identification of a human gene (HCK) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells.
Quintrell N, Lebo R, Varmus H, Bishop JM, Pettenati MJ, Le Beau MM, Diaz MO, Rowley JD.
Mol Cell Biol. 1987 Jun; 7(6):2267-75.
[Mol Cell Biol. 1987]
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