-
A rapid procedure for cloning genes from lambda libraries by complementation of E. coli defective mutants: application to the fabE region of the E. coli chromosome.
Institut de Biologie Physico-Chimique, University of Paris, France.
I describe a general and rapid procedure allowing the isolation of specialized lambda transducing phages from a lambda library by lysogenic complementation of defective mutants of Escherichia coli. As an example, the cloning of the E. coli fabE gene and of two other adjacent genetic determinants is presented. Subcloning and determination of its nucleotide sequence reveals that fabE codes for the biotin carboxyl carrier protein (BCCP), one of the three subunits of acetyl coenzyme A carboxylase.
PMID: 2575489 [PubMed - indexed for MEDLINE]
-
Cited by 9 PubMed Central articles
-
ReviewRegulation of fatty acid biosynthesis in Escherichia coli.
Magnuson K, Jackowski S, Rock CO, Cronan JE Jr.
Microbiol Rev. 1993 Sep; 57(3):522-42.
[Microbiol Rev. 1993]
-
Cotranscription of two genes necessary for ribosomal protein L11 methylation (prmA) and pantothenate transport (panF) in Escherichia coli K-12.
Vanet A, Plumbridge JA, Alix JH.
J Bacteriol. 1993 Nov; 175(22):7178-88.
[J Bacteriol. 1993]
-
Wheat acetyl-coenzyme A carboxylase: cDNA and protein structure.
Gornicki P, Podkowinski J, Scappino LA, DiMaio J, Ward E, Haselkorn R.
Proc Natl Acad Sci U S A. 1994 Jul 19; 91(15):6860-4.
[Proc Natl Acad Sci U S A. 1994]
- » See all...