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The TRP4 gene of Saccharomyces cerevisiae: isolation and structural analysis.
The TRP4 gene of Saccharomyces cerevisiae, encoding the anthranilate phosphoribosyl transferase, was isolated and subcloned by functional complementation in yeast. A 2 kb fragment containing information for a polypeptide of 380 amino acids and the 5'- and 3'-flanking regions was sequenced. The TRP4 transcript was identified and mapped with S1 nuclease. Homologies to two prokaryotic genes encoding the same function, and sequences potentially involved in transcription start and termination and in regulation of TRP4 gene expression are discussed.
PMID: 2428012 [PubMed - indexed for MEDLINE]
PMCID: PMC311651
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Cited by 17 PubMed Central articles
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Replacement of the yeast TRP4 3' untranslated region by a hammerhead ribozyme results in a stable and efficiently exported mRNA that lacks a poly(A) tail.
Düvel K, Valerius O, Mangus DA, Jacobson A, Braus GH.
RNA. 2002 Mar; 8(3):336-44.
[RNA. 2002]
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Sequence requirements of the bidirectional yeast TRP4 mRNA 3'-end formation signal.
Egli CM, Düvel K, Trabesinger-Rüf N, Irniger S, Braus GH.
Nucleic Acids Res. 1997 Jan 15; 25(2):417-22.
[Nucleic Acids Res. 1997]
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Cloning, primary structure, and regulation of the HIS7 gene encoding a bifunctional glutamine amidotransferase: cyclase from Saccharomyces cerevisiae.
Kuenzler M, Balmelli T, Egli CM, Paravicini G, Braus GH.
J Bacteriol. 1993 Sep; 175(17):5548-58.
[J Bacteriol. 1993]
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