-
Human aldolase isozyme gene: the structure of multispecies aldolase B mRNAs.
A complete nucleotide sequence of human aldolase B mRNA was determined with a recombinant cDNA (pHABL120-3). The cDNA insert was composed of 1,652 bases excluding poly(A) tail and the sequence was consistent with the previous results reported by others. However, S1 nuclease mapping and subsequent genomic analysis allowed us to know that the clone possesses two more sites corresponding to 5'-termini in the 5'-noncoding region and another site of polyadenylation in the 3'-noncoding region. In fact, the major aldolase B mRNA species occupying 90% of the total mRNAs initiated at the predominant position corresponding to the position around -82 of the 5'-noncoding sequence in pHABL120-3 and terminated at the distal polyadenylation site. Second species accounting for 9% of the mRNAs initiated at the same site and terminated at the proximal polyadenylation site. The remainings have a longer 5'-noncoding sequence which starts from further upstream region of the major one and pHABL120-3 corresponds to one of these largest clones.
PMID: 2410860 [PubMed - indexed for MEDLINE]
PMCID: PMC321849
-
Cited by 5 PubMed Central articles
-
A panoramic view of gene expression in the human kidney.
Chabardès-Garonne D, Mejéan A, Aude JC, Cheval L, Di Stefano A, Gaillard MC, Imbert-Teboul M, Wittner M, Balian C, Anthouard V, et al.
Proc Natl Acad Sci U S A. 2003 Nov 11; 100(23):13710-5. Epub 2003 Oct 31.
[Proc Natl Acad Sci U S A. 2003]
-
Cloning, sequencing, and characterization of the gene encoding the class I fructose-1,6-bisphosphate aldolase of Staphylococcus carnosus.
Witke C, Götz F.
J Bacteriol. 1993 Nov; 175(22):7495-9.
[J Bacteriol. 1993]
-
Human aldolase A deficiency associated with a hemolytic anemia: thermolabile aldolase due to a single base mutation.
Kishi H, Mukai T, Hirono A, Fujii H, Miwa S, Hori K.
Proc Natl Acad Sci U S A. 1987 Dec; 84(23):8623-7.
[Proc Natl Acad Sci U S A. 1987]
- » See all...