PAPS-reductase of Escherichia coli. Correlating th...[FEBS Lett. 1990]

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1: FEBS Lett. 1990 Jan 15;260(1):6-9. Links

PAPS-reductase of Escherichia coli. Correlating the N-terminal amino acid sequence with the DNA of gene cys H.

Krone FA, Westphal G, Meyer HE, Schwenn JD.

Department of Biology, Ruhr University Bochum, FRG.

The DNA of the gene complementing a PAPS-reductase-deficient strain of Escherichia coli was sequenced. The N-terminal amino acid sequence of the purified PAPS-reductase confirmed that cys H is the structural gene for this enzyme. The open reading frame extends 732 bases and encodes for a peptide of Mr = 27927. The gene product is functionally active when supplemented with thioredoxin and immunologically related with the wild type enzyme.

PMID: 2404794 [PubMed - indexed for MEDLINE]

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