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1: FEBS Lett. 1990 Sep 3;269(2):319-23.Click here to read Links

Isolation and functional expression of pituitary peptidylglycine alpha-amidating enzyme mRNA. A variant lacking the transmembrane domain.

Kato I, Yonekura H, Yamamoto H, Okamoto H.

Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.

We demonstrate that, in rat pituitary, peptidylglycine alpha-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.

PMID: 2401356 [PubMed - indexed for MEDLINE]