Your browser version may not work well with NCBI's Web applications. More information here...
1: Biochem Biophys Res Commun. 1990 Apr 30;168(2):473-8.Click here to read Links

Isolation and characterization of a cDNA for rat liver cysteine dioxygenase.

Hosokawa Y, Matsumoto A, Oka J, Itakura H, Yamaguchi K.

National Institute of Health and Nutrition, Tokyo, Japan.

Cysteine dioxygenase is a key enzyme of cysteine metabolism in mammals. The cDNA clones for rat liver cysteine dioxygenase were isolated by immunological screening and plaque hybridization from a rat liver cDNA library. The longest clone contained an insert of 1458 bp and encoded a polypeptide of 200 amino acids. The clone included the corresponding nucleotide sequence to amino acid sequences obtained from four lysyl endopeptidase-digested fragments of purified rat liver cysteine dioxygenase. The calculated molecular weight of rat liver cysteine dioxygenase was 23,025. Northern blot analysis revealed a single cysteine dioxygenase mRNA species of about 1.7 kb. A computer homology search indicated that this protein showed no homology with any known protein.

PMID: 2334417 [PubMed - indexed for MEDLINE]