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Molecular cloning of cDNA for vitamin D3 25-hydroxylase from rat liver mitochondria.
Department of Biochemistry, Hiroshima University School of Dentistry, Japan.
A cDNA clone encoding mitochondrial vitamin D3 25-hydroxylase was isolated from a rat liver cDNA library by the use of specific antibodies to the enzyme. The isolated cDNA clone was 1.9 kbp long and contained a 1599 bp open reading frame encoding 533 amino acid residues. The deduced primary structure contained a presequence typical for mitochondrial enzymes in the N-terminal region. The N-terminal sequence of the mature enzyme was determined to be Ala-Ile-Pro-Ala-Ala, which agrees perfectly with a portion of the deduced sequence, establishing the cleavage point of the precursor.
PMID: 2318307 [PubMed - indexed for MEDLINE]
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Cited by 8 PubMed Central articles
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27-Oxygenation of C27-sterols and 25-hydroxylation of vitamin D3 in kidney: cloning, structure and expression of pig kidney CYP27A.
Postlind H, Hosseinpour F, Norlin M, Wikvall K.
Biochem J. 2000 Apr 15; 347(Pt 2):349-56.
[Biochem J. 2000]
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Genetics of vitamin D 1alpha-hydroxylase deficiency in 17 families.
Wang JT, Lin CJ, Burridge SM, Fu GK, Labuda M, Portale AA, Miller WL.
Am J Hum Genet. 1998 Dec; 63(6):1694-702.
[Am J Hum Genet. 1998]
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Cloning and expression of rat 25-hydroxyvitamin D3-1alpha-hydroxylase cDNA.
Shinki T, Shimada H, Wakino S, Anazawa H, Hayashi M, Saruta T, DeLuca HF, Suda T.
Proc Natl Acad Sci U S A. 1997 Nov 25; 94(24):12920-5.
[Proc Natl Acad Sci U S A. 1997]
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