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Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies.
Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702-1201.
A 2.2 kb cDNA clone which presumably includes the complete human B-raf coding sequence was isolated from a human testes cDNA library. Sequence analysis showed the presence of all three conserved regions CR1, CR2 and CR3 previously identified in raf protein kinases. The cDNA was expressed in E. coli yielding a B-raf fusion protein which reacts with antibodies specific for B-raf raised against the C-terminal peptide as well as with monoclonal antibodies which map into the kinase domain.
PMID: 2284096 [PubMed - indexed for MEDLINE]
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Cited by 15 PubMed Central articles
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Oncogenic AKAP9-BRAF fusion is a novel mechanism of MAPK pathway activation in thyroid cancer.
Ciampi R, Knauf JA, Kerler R, Gandhi M, Zhu Z, Nikiforova MN, Rabes HM, Fagin JA, Nikiforov YE.
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[J Clin Invest. 2005]
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Constitutive activation of the Ras-Raf signaling pathway in metastatic melanoma is associated with poor prognosis.
Houben R, Becker JC, Kappel A, Terheyden P, Bröcker EB, Goetz R, Rapp UR.
J Carcinog. 2004 Mar 26; 3(1):6. Epub 2004 Mar 26.
[J Carcinog. 2004]
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The strength of interaction at the Raf cysteine-rich domain is a critical determinant of response of Raf to Ras family small GTPases.
Okada T, Hu CD, Jin TG, Kariya K, Yamawaki-Kataoka Y, Kataoka T.
Mol Cell Biol. 1999 Sep; 19(9):6057-64.
[Mol Cell Biol. 1999]
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