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The metalloprotease gene of Serratia marcescens strain SM6.
Department of Biology, Texas A & M University, College Station 77843-3258.
Utilizing the DNA sequence of the metalloprotease from Serratia strain E-15, we isolated and sequenced the homologous gene from Serratia strain SM6. These two genes are similar at both the DNA and protein sequence level. Expression of the protease gene in Escherichia coli was achieved by use of the lac promoter. This resulted in the production and excretion of an immunologically detectable but inactive protein of slightly higher molecular weight than that from Serratia. We introduced the cloned gene into previously described protease mutants. The observed pattern of protease expression suggested that these mutations fall into three classes.
PMID: 2274043 [PubMed - indexed for MEDLINE]
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Cited by 8 PubMed Central articles
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Subset of hybrid eukaryotic proteins is exported by the type I secretion system of Erwinia chrysanthemi.
Palacios JL, Zaror I, Martínez P, Uribe F, Opazo P, Socías T, Gidekel M, Venegas A.
J Bacteriol. 2001 Feb; 183(4):1346-58.
[J Bacteriol. 2001]
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ReviewMolecular and biotechnological aspects of microbial proteases.
Rao MB, Tanksale AM, Ghatge MS, Deshpande VV.
Microbiol Mol Biol Rev. 1998 Sep; 62(3):597-635.
[Microbiol Mol Biol Rev. 1998]
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Polymerase chain reaction selects a novel disintegrin proteinase from CD40-activated germinal center dendritic cells.
Mueller CG, Rissoan MC, Salinas B, Ait-Yahia S, Ravel O, Bridon JM, Briere F, Lebecque S, Liu YJ.
J Exp Med. 1997 Aug 29; 186(5):655-63.
[J Exp Med. 1997]
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