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1: Gene. 1990 Jul 2;91(1):27-34.Click here to read Links

The methane monooxygenase gene cluster of Methylococcus capsulatus (Bath).

Stainthorpe AC, Lees V, Salmond GP, Dalton H, Murrell JC.

Department of Biological Sciences, University of Warwick, Coventry, U.K.

Methane is oxidised to methanol in methanotrophic bacteria by the enzyme methane monooxygenase (MMO). Methylococcus capsulatus (Bath) produces a soluble MMO which oxidises a range of aliphatic and aromatic compounds with potential for commercial exploitation. This multicomponent enzyme has been extensively characterised and biochemical data have been used to identify a 12-kb fragment of Methylococcus DNA carrying the structural genes mmoY and mmoZ, coding for the beta- and gamma-subunits of MMO component A, the methane-binding protein. We now report the complete nucleotide (nt) sequence of mmoX, the gene encoding the alpha-subunit of component A which is found to be 5' to mmoY and mmoZ. We also report the complete nt sequence of mmoC which encodes component C, the iron-sulfur flavoprotein of MMO, the N terminus of which is significantly homologous with spinach ferredoxin. The mmo structural genes are clustered within a 7-kb region and are closely linked to two small open reading frames of unknown function.

PMID: 2205538 [PubMed - indexed for MEDLINE]