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Mapping the active site of yeast RNA polymerase B (II).
Département de Biologie, Centre d'Etudes Nucléaires de Saclay, Gif-sur-Yvette, France.
Yeast RNA polymerase B (II) was incubated with a collection of 13 different nucleotide derivatives and affinity labeled by allowing DNA-directed phosphodiester bond formation. The 32P-labeled site was localized in the C-terminal part of the B150 subunit by microsequencing a proteolytic fragment, then further mapped by a combination of extensive or single-hit chemical cleavage reactions and analysis of the labeled peptide patterns. The affinity label was mapped to between Asn946 and Met999, within one of the nine regions that are conserved between B150 and the bacterial beta subunit. The results underscore the conservative evolution of the catalytic center of eukaryotic and bacterial RNA polymerases.
PMID: 2204624 [PubMed - indexed for MEDLINE]
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Cited by 13 PubMed Central articles
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The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits.
Peyroche G, Levillain E, Siaut M, Callebaut I, Schultz P, Sentenac A, Riva M, Carles C.
Proc Natl Acad Sci U S A. 2002 Nov 12; 99(23):14670-5. Epub 2002 Oct 29.
[Proc Natl Acad Sci U S A. 2002]
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RNA-binding site in T7 RNA polymerase.
Sastry S, Ross BM.
Proc Natl Acad Sci U S A. 1998 Aug 4; 95(16):9111-6.
[Proc Natl Acad Sci U S A. 1998]
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A34.5, a nonessential component of yeast RNA polymerase I, cooperates with subunit A14 and DNA topoisomerase I to produce a functional rRNA synthesis machine.
Gadal O, Mariotte-Labarre S, Chedin S, Quemeneur E, Carles C, Sentenac A, Thuriaux P.
Mol Cell Biol. 1997 Apr; 17(4):1787-95.
[Mol Cell Biol. 1997]
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