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Grapevine stilbene synthase cDNA only slightly differing from chalcone synthase cDNA is expressed in Escherichia coli into a catalytically active enzyme.
University of Marburg, Department of Chemistry, FRG.
Stilbene synthase is responsible for the formation of resveratrol and other stilbenes which function in grapevine as phytoalexins. A full-length stilbene synthase cDNA was prepared from grapevine mRNA and sequenced. The insert in pSV25 coding for a polypeptide with 392 amino acids was inserted into the vectors pKK233-2 and pDS12/RBSII-2, respectively. Expression of the cDNA in Escherichia coli yielded an enzymatically active dimer exhibiting solely stilbene synthase activity. The protein was characterized by enzyme activity and Western blot analysis.
PMID: 2200709 [PubMed - indexed for MEDLINE]
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Cited by 5 PubMed Central articles
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Complete genome sequence of the soil actinomycete Kocuria rhizophila.
Takarada H, Sekine M, Kosugi H, Matsuo Y, Fujisawa T, Omata S, Kishi E, Shimizu A, Tsukatani N, Tanikawa S, et al.
J Bacteriol. 2008 Jun; 190(12):4139-46. Epub 2008 Apr 11.
[J Bacteriol. 2008]
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Flavonoid Evolution: An Enzymic Approach.
Stafford HA.
Plant Physiol. 1991 Jul; 96(3):680-685.
[Plant Physiol. 1991]
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A stilbene synthase from Japanese red pine (Pinus densiflora): implications for phytoalexin accumulation and down-regulation of flavonoid biosynthesis.
Kodan A, Kuroda H, Sakai F.
Proc Natl Acad Sci U S A. 2002 Mar 5; 99(5):3335-9.
[Proc Natl Acad Sci U S A. 2002]
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