Prokaryotic 20 beta-hydroxysteroid dehydrogenase i...[FEBS Lett. 1990]

SearchDatabase nameforSearch term

Advanced Search

Your browser version may not work well with NCBI's Web applications. More information here...

Display Show

All: 1

Review: 0

Click to change filter selection through MyNCBI.

1: FEBS Lett. 1990 Jun 18;266(1-2):51-4. Links

Prokaryotic 20 beta-hydroxysteroid dehydrogenase is an enzyme of the 'short-chain, non-metalloenzyme' alcohol dehydrogenase type.

Marekov L, Krook M, Jörnvall H.

Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden.

The primary structure of 20 beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was determined after FPLC purification of a commercial preparation. Peptides obtained from different proteolytic cleavages were purified by reverse phase HPLC. The 255-residue structure deduced was found to be distantly homologous to those of Drosophila alcohol dehydrogenase and several other dehydrogenases, establishing that prokaryotic 20 beta-hydroxysteroid dehydrogenase as a member of the 'short-chain alcohol dehydrogenase family'. With the enzymes characterized, the identity is greatest (31-34%) towards 4 other prokaryotic dehydrogenases, but the family also includes mammalian steroid and prostaglandin dehydrogenases. These enzymes are low in Cys and have a strictly conserved Tyr residue that appears to be important.

PMID: 2194840 [PubMed - indexed for MEDLINE]

Pseudomonas 3 beta-hydroxysteroid dehydrogenase. Primary structure and relationships to other steroid dehydrogenases.
Eur J Biochem. 1991 Apr 23; 197(2):359-65.

[Eur J Biochem. 1991]
Short-chain dehydrogenases. Proteolysis and chemical modification of prokaryotic 3 alpha/20 beta-hydroxysteroid, insect alcohol and human 15-hydroxyprostaglandin dehydrogenases.
Eur J Biochem. 1992 Oct 1; 209(1):233-9.

[Eur J Biochem. 1992]
Comparison of the 3alpha-and 20beta-hydroxysteroid dehydrogenase activities of the cortisone reductase of Streptomyces hydrogenans.
Biochemistry. 1978 Oct 17; 17(21):4370-6.

[Biochemistry. 1978]
ReviewSteroid dehydrogenase structures, mechanism of action, and disease.
Vitam Horm. 2000; 58:121-48.

[Vitam Horm. 2000]
ReviewThe short-chain alcohol dehydrogenase superfamily: variations on a common theme.
J Steroid Biochem Mol Biol. 1994 Nov; 51(3-4):125-30.

[J Steroid Biochem Mol Biol. 1994]
» See reviews... | » See all...

Cited by 14 PubMed Central articles

2-Hydroxycyclohexanecarboxyl coenzyme A dehydrogenase, an enzyme characteristic of the anaerobic benzoate degradation pathway used by Rhodopseudomonas palustris.
Pelletier DA, Harwood CS. J Bacteriol. 2000 May; 182(10):2753-60.

[J Bacteriol. 2000]
Cloning, expression, and molecular characterization of a small pea gene family regulated by low levels of ultraviolet B radiation and other stresses.
Brosché M, Strid A. Plant Physiol. 1999 Oct; 121(2):479-87.

[Plant Physiol. 1999]
Cloning, nucleotide sequence, and overexpression of smoS, a component of a novel operon encoding an ABC transporter and polyol dehydrogenases of Rhodobacter sphaeroides Si4.
Stein MA, Schäfer A, Giffhorn F. J Bacteriol. 1997 Oct; 179(20):6335-40.

[J Bacteriol. 1997]
» See all...

Recent Activity

Clear Turn Off Turn On

Prokaryotic 20 beta-hydroxysteroid dehydrogenase is an enzyme of the 'short-chain, non-met...Prokaryotic 20 beta-hydroxysteroid dehydrogenase is an enzyme of the 'short-chain, non-metalloenzyme' alcohol dehydrogenase type.

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

Display Show

Prokaryotic 20 beta-hydroxysteroid dehydrogenase is an enzyme of the 'short-chain, non-metalloenzyme' alcohol dehydrogenase type.

Related articles

Cited by 14 PubMed Central articles

Recent Activity