Cloning and characterization of the iron-sulfur su...[J Biol Chem. 1990]

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1: J Biol Chem. 1990 Jun 25;265(18):10419-23. Links

Cloning and characterization of the iron-sulfur subunit gene of succinate dehydrogenase from Saccharomyces cerevisiae.

Lombardo A, Carine K, Scheffler IE.

Department of Biology, University of California, San Diego, La Jolla 92093.

We describe the cloning and characterization of the complete gene for the iron-sulfur protein subunit of succinate dehydrogenase (EC 1.3.99.1) from Saccharomyces cerevisiae. The promoter and coding sequence have been cloned into an Escherichia coli-yeast shuttle vector. The cloned gene complements the defect in a succinate dehydrogenase-deficient yeast mutant isolated by us, and gene expression is fully responsive to induction by glucose deprivation, indicating that the promoter is intact.

PMID: 2191948 [PubMed - indexed for MEDLINE]

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Cloning and characterization of the iron-sulfur subunit gene of succinate dehydrogenase from Saccharomyces cerevisiae.

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Cited by 8 PubMed Central articles

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