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Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-beta-lipotropin precursor.
mRNA that encodes the common peptide precursor for the hormones corticotropin and beta-lipotropin was purified from the neurointermediate lobe of bovine pituitaries, and double-stranded cDNA species synthesized from this template were cloned in Escherichia coli X1776 by inserting them into the Pst I endonuclease cleavage site of the pBR322 plasmid using poly(dG)poly(dC) homopolymeric extensions. Certain of the cloned cDNA inserts contain nucleotides corresponding to the complete amino acid sequence of bovine corticotropin and a coding sequence that corresponds to at least the first portion of bovine beta-lipotropin. The nucleotide sequences coding for corticotropin and beta-lipotropin are separated on the cDNA by a 6-base-pair sequence encoding lysine and arginine, indicating that the carboxyl terminus of corticotropin is connected on the precursor peptide with the amino terminus of beta-lipotropin by these two amino acids. In addition, the cloned cDNA insert is characterized by an unusually high C+G nucleotide base content as well as by a number of DNA sequence duplications.
PMID: 216007 [PubMed - indexed for MEDLINE]
PMCID: PMC393109
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Cited by 6 PubMed Central articles
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Characterization of the beta-lactamase promoter of pBR322.
Russell DR, Bennett GN.
Nucleic Acids Res. 1981 Jun 11; 9(11):2517-33.
[Nucleic Acids Res. 1981]
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Structural organization of human genomic DNA encoding the pro-opiomelanocortin peptide.
Chang AC, Cochet M, Cohen SN.
Proc Natl Acad Sci U S A. 1980 Aug; 77(8):4890-4.
[Proc Natl Acad Sci U S A. 1980]
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Isolation, structure, biological characterization, and synthesis of beta-[Tyr9]melanotropin-(9-18) decapeptide from pig hypothalami.
Schally AV, Chang RC, Huang WY, Coy DH, Kastin AJ, Redding TW.
Proc Natl Acad Sci U S A. 1980 Jul; 77(7):3947-51.
[Proc Natl Acad Sci U S A. 1980]
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