-
Rat liver NAD(P)H:quinone oxidoreductase: cDNA expression and site-directed mutagenesis.
Department of Biology, Beckman Research Institute/City of Hope, Duarte, CA 91010.
Rat liver NAD(P)H:quinone oxidoreductase cDNA was cloned and expressed in a eukaryotic cell expression plasmid containing a cytomegalovirus (CMV) promoter. Transient expression of enzyme activity and RNA transcription were measured in COS7 cells. The expressed quinone reductase has kinetic properties similar to the rat liver enzyme and is inhibited by dicourmarol, a known inhibitor of NAD(P)H:quinone oxidoreductase. Site-directed mutagenesis experiments carried out using this expression system revealed possible regions involved in NAD(P)H binding.
PMID: 2141979 [PubMed - indexed for MEDLINE]
-
Cited by 3 PubMed Central articles
-
Mouse liver NAD(P)H:quinone acceptor oxidoreductase: protein sequence analysis by tandem mass spectrometry, cDNA cloning, expression in Escherichia coli, and enzyme activity analysis.
Chen S, Clarke PE, Martino PA, Deng PS, Yeh CH, Lee TD, Prochaska HJ, Talalay P.
Protein Sci. 1994 Aug; 3(8):1296-304.
[Protein Sci. 1994]
-
A two-domain structure for the two subunits of NAD(P)H:quinone acceptor oxidoreductase.
Chen S, Deng PS, Bailey JM, Swiderek KM.
Protein Sci. 1994 Jan; 3(1):51-7.
[Protein Sci. 1994]
-
Expression of rat liver NAD(P)H:quinone-acceptor oxidoreductase in Escherichia coli and mutagenesis in vitro at Arg-177.
Chen HH, Ma JX, Forrest GL, Deng PS, Martino PA, Lee TD, Chen S.
Biochem J. 1992 Jun 15; 284 ( Pt 3):855-60.
[Biochem J. 1992]