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1: J Biol Chem. 1990 Nov 15;265(32):19932-6.Click here to read Links

Androgen-dependent protein from mouse vas deferens. cDNA cloning and protein homology with the aldo-keto reductase superfamily.

Pailhoux EA, Martinez A, Veyssiere GM, Jean CG.

Physiologie Comparée et Endocrinologie, Centre National de la Recherche Scientifique U.A. 360, Université Blaise Pascal, Les Cézeaux, Aubière France.

We report the cloning and sequencing of a 1225-base pair cDNA encoding an abundant protein from mouse vas deferens. An open reading frame of 948 nucleotides encodes a protein of 316 amino acids with a calculated mass of 35,965 Da. A high degree of homology was found between this protein and members of the aldo-keto reductase superfamily, especially aldose reductase from human placenta (82%) and from rat lens (80%), suggesting that it could be an aldose reductase. Castration resulted in a marked decrease in the level of the 1.4 kilobase mRNA coding for the 35,965 protein, whereas administration of testosterone to castrated males resulted in a marked increase. Southern hybridization analysis of mouse genomic DNA revealed relatively simple patterns of bands.

PMID: 2123194 [PubMed - indexed for MEDLINE]