Your browser version may not work well with NCBI's Web applications. More information here...
1: Biochem Biophys Res Commun. 1990 Mar 30;167(3):948-55.Click here to read Links

Structure and expression of cysX, the second gene in the Escherichia coli K-12 cysE locus.

Tei H, Murata K, Kimura A.

Research Institute for Food Science, Kyoto University, Japan.

Protein products of the cysE region at 81 min on the chromosome of Escherichia coli K-12 (1) were analyzed by the maxicell method. Two kinds of polypeptides of molecular weight 33 K and 16 K were the products. The open reading frame (ORF) of the 33 K polypeptide consisted of 273 amino acids (Mr = 29,261). On the other hand, the 16 K ORF was overlapped by the opposite 33 K ORF and specified an extremely basic protein of 130 amino acids (Mr = 15,233). The gene coding for the 16 K polypeptide was named cysX. The expression of cysE and cysX in vivo was confirmed further by constructions of the cysE'-'lacZ and cysX'-'lacZ hybrid genes.

PMID: 2108679 [PubMed - indexed for MEDLINE]