Evidence that the protein components of bovine erythrocyte green heme binding protein and flavin reductase are identical.

Department of Biological Chemistry, Medical School, University of Michigan, Ann Arbor 48109-0606.

Bovine erythrocyte green heme binding protein and bovine erythrocyte flavin reductase have been isolated in highly purified forms and subjected to amino acid analysis and N-terminal amino acid sequence analysis. The two proteins possess similar amino acid compositions and identical N-terminal amino acid sequences. Moreover, the two proteins are immunochemically cross-reactive and are indistinguishable when compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by double diffusion technique. This study provides evidence that the protein components of bovine erythrocyte green heme binding protein and flavin reductase are identical.

PMID: 2069570 [PubMed - indexed for MEDLINE]