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Mitochondrial protein import: isolation and characterization of the Saccharomyces cerevisiae MFT1 gene.
Department of Biology, Hamilton College, Clinton, NY 13323.
Mitochondrial targeting of an Atp2-LacZ fusion protein confers a respiration-defective phenotype on yeast cells. This effect has been utilized to select strains that grow on nonfermentable carbon sources, some of which have decreased levels of hybrid protein localized to the organelle. Many of the mutants obtained were also temperature-sensitive for growth on all media. The recessive mft (mitochondrial fusion targeting) mutants have been assigned to three complementation groups. MFT1 was cloned and sequenced: it encodes a 255 amino acid protein that is highly basic and has no predicted membrane-spanning domains or organelle-targeting sequences. The MFT1 gene is 91% identical to an open reading frame 3' of the SIR3 gene. Evidence is presented that these two closely related genes could represent a recent gene duplication.
PMID: 2017143 [PubMed - indexed for MEDLINE]
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Cited by 8 PubMed Central articles
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A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae.
Chávez S, Beilharz T, Rondón AG, Erdjument-Bromage H, Tempst P, Svejstrup JQ, Lithgow T, Aguilera A.
EMBO J. 2000 Nov 1; 19(21):5824-34.
[EMBO J. 2000]
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Two mutant forms of the S1/TPR-containing protein Rrp5p affect the 18S rRNA synthesis in Saccharomyces cerevisiae.
Torchet C, Jacq C, Hermann-Le Denmat S.
RNA. 1998 Dec; 4(12):1636-52.
[RNA. 1998]
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Altered cellular responses by varying expression of a ribosomal protein gene: sequential coordination of enhancement and suppression of ribosomal protein S3a gene expression induces apoptosis.
Naora H, Takai I, Adachi M, Naora H.
J Cell Biol. 1998 May 4; 141(3):741-53.
[J Cell Biol. 1998]
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