Sequencing and expression of the rne gene of Esche...[Nucleic Acids Res. 1991]

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1: Nucleic Acids Res. 1991 Jan 11;19(1):125-9. Links

Sequencing and expression of the rne gene of Escherichia coli.

Chauhan AK, Miczak A, Taraseviciene L, Apirion D.

Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110.

RNase E is a major endonucleolytic RNA processing enzyme in Escherichia coli. We have sequenced a 3.2 kb EcoRI-BamHI fragment encoding the rne gene, and identified its reading frame. Upstream from the gene, there are appropriate consensus sequences for a putative promoter and a ribosome binding site. We have translated this gene using a T7 RNA polymerase/promoter system. We determined 25 amino acids from the N-terminal of the translated product and they are in full agreement with the DNA sequence. The translated product of the rne gene migrates in SDS containing polyacrylamide gels as a 110,000 Da polypeptide, but the open reading frame found in the sequenced DNA indicates a much smaller protein. The entity that migrates as a 110,000 Da contains RNA, which could account, at least partially, for the migration of the rne gene product in SDS containing polyacrylamide gels.

PMID: 2011493 [PubMed - indexed for MEDLINE]

PMCID: PMC333542

Evidence for an RNA binding region in the Escherichia coli processing endoribonuclease RNase E.
J Biol Chem. 1995 Nov 3; 270(44):26391-8.

[J Biol Chem. 1995]
Immunoaffinity purification of the Escherichia coli rne gene product. Evidence that the rne gene encodes the processing endoribonuclease RNase E.
J Biol Chem. 1994 Apr 22; 269(16):12167-72.

[J Biol Chem. 1994]
RNase E activity is conferred by a single polypeptide: overexpression, purification, and properties of the ams/rne/hmp1 gene product.
Proc Natl Acad Sci U S A. 1993 Oct 1; 90(19):9006-10.

[Proc Natl Acad Sci U S A. 1993]
DNA sequencing and expression of the gene rnb encoding Escherichia coli ribonuclease II.
Mol Microbiol. 1993 Apr; 8(1):43-51.

[Mol Microbiol. 1993]
RNase E levels in Escherichia coli are controlled by a complex regulatory system that involves transcription of the rne gene from three promoters.
Mol Microbiol. 2002 Jan; 43(1):159-71.

[Mol Microbiol. 2002]
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Cited by 6 PubMed Central articles

ReviewLinkage map of Escherichia coli K-12, edition 10: the traditional map.
Berlyn MK. Microbiol Mol Biol Rev. 1998 Sep; 62(3):814-984.

[Microbiol Mol Biol Rev. 1998]
RNase E activity is conferred by a single polypeptide: overexpression, purification, and properties of the ams/rne/hmp1 gene product.
Cormack RS, Genereaux JL, Mackie GA. Proc Natl Acad Sci U S A. 1993 Oct 1; 90(19):9006-10.

[Proc Natl Acad Sci U S A. 1993]
The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus.
McDowall KJ, Hernandez RG, Lin-Chao S, Cohen SN. J Bacteriol. 1993 Jul; 175(13):4245-9.

[J Bacteriol. 1993]
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