Crystal structure of recombinant human T-cell cycl...[Proc Natl Acad Sci U S A. 1991]

SearchDatabase nameforSearch term

Advanced Search

Your browser version may not work well with NCBI's Web applications. More information here...

Display Show

All: 1

Review: 0

Click to change filter selection through MyNCBI.

1: Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9483-7. Links

Crystal structure of recombinant human T-cell cyclophilin A at 2.5 A resolution.

Ke HM, Zydowsky LD, Liu J, Walsh CT.

Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599.

The structure of the unligated human T-cell recombinant cyclophilin has been determined at 3 A resolution by multipole isomorphous replacement methods and refined at 2.5 A resolution to an R factor of 0.209. The root-mean-square errors of the bond lengths and bond angles are 0.013 A and 2.8 degrees from ideal geometry, respectively. The overall structure is a beta-barrel, consisting of eight antiparallel beta-strands wrapping around the barrel surface and two alpha-helices sitting on the top and the bottom closing the barrel. Inside the barrel, seven aromatic and other hydrophobic residues form a compact hydrophobic core. A loop of Lys-118 to His-126 and four beta-strands (B3-B6) constitute a pocket we speculate to be the binding site of cyclosporin A.

PMID: 1946361 [PubMed - indexed for MEDLINE]

PMCID: PMC52742

Similarities and differences between human cyclophilin A and other beta-barrel structures. Structural refinement at 1.63 A resolution.
J Mol Biol. 1992 Nov 20; 228(2):539-50.

[J Mol Biol. 1992]
Structure of human cyclophilin and its binding site for cyclosporin A determined by X-ray crystallography and NMR spectroscopy.
Nature. 1991 Sep 19; 353(6341):276-9.

[Nature. 1991]
Crystal structure of cyclophilin A complexed with substrate Ala-Pro suggests a solvent-assisted mechanism of cis-trans isomerization.
Proc Natl Acad Sci U S A. 1993 Apr 15; 90(8):3324-8.

[Proc Natl Acad Sci U S A. 1993]
X-ray structure of a cyclophilin B/cyclosporin complex: comparison with cyclophilin A and delineation of its calcineurin-binding domain.
Proc Natl Acad Sci U S A. 1994 May 24; 91(11):5183-6.

[Proc Natl Acad Sci U S A. 1994]
ReviewThe crystal structure of glutamine-binding protein from Escherichia coli.
J Mol Biol. 1996 Sep 20; 262(2):225-42.

[J Mol Biol. 1996]
» See reviews... | » See all...

Cited by 19 PubMed Central articles

Induction of G2 arrest and binding to cyclophilin A are independent phenotypes of human immunodeficiency virus type 1 Vpr.
Ardon O, Zimmerman ES, Andersen JL, DeHart JL, Blackett J, Planelles V. J Virol. 2006 Apr; 80(8):3694-700.

[J Virol. 2006]
ReviewThe cyclophilins.
Wang P, Heitman J. Genome Biol. 2005; 6(7):226. Epub 2005 Jun 27.

[Genome Biol. 2005]
ReviewPeptidyl-prolyl isomerases: a new twist to transcription.
Shaw PE. EMBO Rep. 2002 Jun; 3(6):521-6.

[EMBO Rep. 2002]
» See all...

Structures reported by this article

Similarities And Differences Between Human Cyclophilin A And Other Beta-Barrel Structures. Structural Refinement At 1.63 Angstroms Resolution

PDB: 2CPL

Source: Homo sapiens

Method: X-Ray Diffraction | Resolution: 1.63 Å

Patient Drug Information

Cyclosporine (Neoral® , Sandimmune® , Gengraf® )
Cyclosporine and cyclosporine (modified) are used with other medications to prevent transplant rejection (attack of the transplanted organ by the immune system of the person who received the organ) in people who have rec...
Source: AHFS Consumer Medication Information

Recent Activity

Clear Turn Off Turn On

Crystal structure of recombinant human T-cell cyclophilin A at 2.5 A resolution.Crystal structure of recombinant human T-cell cyclophilin A at 2.5 A resolution.

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on