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Molecular analysis of the Salmonella typhimurium phoN gene, which encodes nonspecific acid phosphatase.
Department of Experimental Chemotherapy, Osaka University, Japan.
The phoN gene of Salmonella typhimurium encodes nonspecific acid phosphatase (EC 3.1.3.2), which is regulated by a two-component regulatory system consisting of the phoP and phoQ genes. We cloned the phoN region into a plasmid vector by complementation of a phoN mutant strain and determined the nucleotide sequence of the phoN gene and its flanking regions. The phoN gene could encode a 26-kDa protein, which was identified by the maxicell method as the product of phoN. Results of the enzyme assay and Southern hybridization with chromosomal DNA of Escherichia coli K-12 suggests that there is no phoN gene in E. coli. The regulatory pattern of phoN in E. coli and Southern hybridization analysis of the E. coli chromosome with the S. typhimurium phoP gene suggest that E. coli K-12 also harbors the phoP and phoQ genes.
PMID: 1938882 [PubMed - indexed for MEDLINE]
PMCID: PMC209025
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Cited by 11 PubMed Central articles
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The Xanthomonas oryzae pv. oryzae PhoPQ two-component system is required for AvrXA21 activity, hrpG expression, and virulence.
Lee SW, Jeong KS, Han SW, Lee SE, Phee BK, Hahn TR, Ronald P.
J Bacteriol. 2008 Mar; 190(6):2183-97. Epub 2008 Jan 18.
[J Bacteriol. 2008]
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X-ray structures of a novel acid phosphatase from Escherichia blattae and its complex with the transition-state analog molybdate.
Ishikawa K, Mihara Y, Gondoh K, Suzuki E, Asano Y.
EMBO J. 2000 Jun 1; 19(11):2412-23.
[EMBO J. 2000]
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Isolation, cloning, and expression of an acid phosphatase containing phosphotyrosyl phosphatase activity from Prevotella intermedia.
Chen X, Ansai T, Awano S, Iida T, Barik S, Takehara T.
J Bacteriol. 1999 Nov; 181(22):7107-14.
[J Bacteriol. 1999]
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