Cloning of the D-lactate dehydrogenase gene from L...[FEBS Lett. 1991]

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1: FEBS Lett. 1991 Sep 23;290(1-2):61-4. Links

Cloning of the D-lactate dehydrogenase gene from Lactobacillus delbrueckii subsp. bulgaricus by complementation in Escherichia coli.

Bernard N, Ferain T, Garmyn D, Hols P, Delcour J.

Laboratoire de Génétique Moléculaire, Université Catholique de Louvain, Louvain-la Neuve, Belgium.

A strain of Escherichia coli (FMJ144) deficient for pyruvate formate lyase and lactate dehydrogenase (LDH) was complemented with a genomic DNA library from Lactobacillus delbrueckii subsp. bulgaricus. One positive cloned showed LDH activity and production of D(-)lactate was demonstrated. The nucleotide sequence of the D-LDH gene (ldhA) revealed the spontaneous insertion of an E. coli insertion sequence IS2 upstream of the gene coding region. The open reading frame encoded a 333-amino acid protein, showing no similarity with known L-LDH sequences but closely related to L. casei D-hydroxyisocaproate dehydrogenase (D-HicDH).

PMID: 1915894 [PubMed - indexed for MEDLINE]

NAD(+)-dependent D-2-hydroxyisocaproate dehydrogenase of Lactobacillus delbrueckii subsp. bulgaricus. Gene cloning and enzyme characterization.
Eur J Biochem. 1994 Sep 1; 224(2):439-46.

[Eur J Biochem. 1994]
Cloning and overexpression of Lactobacillus helveticus D-lactate dehydrogenase gene in Escherichia coli.
Eur J Biochem. 1992 Sep 15; 208(3):799-805.

[Eur J Biochem. 1992]
D-lactate dehydrogenase is a member of the D-isomer-specific 2-hydroxyacid dehydrogenase family. Cloning, sequencing, and expression in Escherichia coli of the D-lactate dehydrogenase gene of Lactobacillus plantarum.
J Biol Chem. 1991 Jul 5; 266(19):12588-94.

[J Biol Chem. 1991]
Cloning and overexpression of the Lactobacillus bulgaricus NAD(+)-dependent D-lactate dehydrogenase gene in Escherichia coli:purification and characterization of the recombinant enzyme.
Biochem Biophys Res Commun. 1992 Jun 15; 185(2):705-12.

[Biochem Biophys Res Commun. 1992]
Review[Lactate dehydrogenase subunit deficiencies]
Seikagaku. 1992 Jun; 64(6):401-4.

[Seikagaku. 1992]
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Cloning of the D-lactate dehydrogenase gene from Lactobacillus delbrueckii subsp. bulgaricus by complementation in Escherichia coli.

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Cited by 17 PubMed Central articles

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