Identification of potential amino acid residues su...[FEBS Lett. 1991]

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1: FEBS Lett. 1991 Sep 9;289(2):217-20. Links

Identification of potential amino acid residues supporting anticodon recognition in yeast methionyl-tRNA synthetase.

Despons L, Walter P, Senger B, Ebel JP, Fasiolo F.

Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.

Sequence comparisons among methionyl-tRNA synthetases from different organisms reveal only one block of homology beyond the last beta strand of the mononucleotide fold. We have introduced a series of semi-conservative amino acid replacements in the conserved motif of yeast methionyl-tRNA synthetase. The results indicate that replacements of two polar residues (Asn584 and Arg588) affected specifically the aminoacylation reaction. The location of these residues in the tertiary structure of the enzyme is compatible with a direct interaction of the amino acid side-chains with the tRNA anticodon.

PMID: 1915850 [PubMed - indexed for MEDLINE]

Binding of the yeast tRNA(Met) anticodon by the cognate methionyl-tRNA synthetase involves at least two independent peptide regions.
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Yeast methionyl-tRNA synthetase: analysis of the N-terminal extension and the putative tRNA anticodon binding region by site-directed mutagenesis.
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[Biochimie. 1990]
ReviewYeast tRNA(Met) recognition by methionyl-tRNA synthetase requires determinants from the primary, secondary and tertiary structure: a review.
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[Biochimie. 1996]
Review[Pleitropic resistance in yeast and its connection with multiple resistance in higher eukaryotes]
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[Mol Biol (Mosk). 1992]
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Cited by 3 PubMed Central articles

The A1 x U72 base pair conserved in eukaryotic initiator tRNAs is important specifically for binding to the eukaryotic translation initiation factor eIF2.
Farruggio D, Chaudhuri J, Maitra U, RajBhandary UL. Mol Cell Biol. 1996 Aug; 16(8):4248-56.

[Mol Cell Biol. 1996]
Single sequence of a helix-loop peptide confers functional anticodon recognition on two tRNA synthetases.
Auld DS, Schmimmel P. EMBO J. 1996 Mar 1; 15(5):1142-8.

[EMBO J. 1996]
RNA binding determinant in some class I tRNA synthetases identified by alignment-guided mutagenesis.
Shepard A, Shiba K, Schimmel P. Proc Natl Acad Sci U S A. 1992 Oct 15; 89(20):9964-8.

[Proc Natl Acad Sci U S A. 1992]

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Identification of potential amino acid residues supporting anticodon recognition in yeast methionyl-tRNA synthetase.

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Cited by 3 PubMed Central articles

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